Supplementary Figure 3.

The effect of butyrate on the expression of Foxp3 in the colons. Control: Control group, SAP: SAP group, SAP + butyrate: SAP + butyrate group. (A) The immunofluorescence detection of Foxp3 in the colon tissues. Red particles (arrow) represented the positive change. (B) The quantitative analysis of immunofluorescence detection of Foxp3 in the colon tissues. The quantitative analysis of immunofluorescence was by ImageJ software. Control group versus SAP group: P < .001, Control group versus SAP + butyrate group: P < .05, SAP group versus SAP + butyrate group: P < .001. n = 8 in each group. SAP, severe acute pancreatitis.