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. Author manuscript; available in PMC: 2022 Sep 30.
Published in final edited form as: Science. 2022 Sep 22;377(6613):1413–1419. doi: 10.1126/science.abo4837

Fig. 1. Macrophages make intimate interactions with newly formed HSPCs.

Fig. 1.

(A) Time lapse live-imaging identifies prolonged cell-cell contacts between runx1+23:EGFP+ HSPCs and mpeg1:mCherry+ primitive macrophages involving exchange of fluorescent material. (B) Approximately 20–30% of HSPCs interact with macrophages in the CHT at any one time from 56 hpf to 106 hpf. Mean +/− s.d. (C) High-resolution tracking of individual runx1+23:mCherry+ cells over several hours in the CHT reveals that the majority of HSPCs eventually make sustained contact with macrophages (> 5 minutes). Mean +/− s.d. (D) HSPCs frequently complete a cell division shortly after macrophage interactions. Approximately 81% of HSPC divisions occur within 30 minutes of a macrophage interaction. Mean +/− s.d. (E) Around 65% of Fucci+ HSPCs in S/G2/M phases interact with macrophages at any one time, as compared to less than 20% of Fucci− HSPCs. Mean +/− s.d., Unpaired t test; ****P<0.0001.