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. Author manuscript; available in PMC: 2022 Sep 30.
Published in final edited form as: Science. 2022 Sep 22;377(6613):1413–1419. doi: 10.1126/science.abo4837

Fig. 2. Macrophages in the CHT regulate stem cell clonality.

Fig. 2.

(A) A schematic overview of the Zebrabow-M system: animals with 15–20 insertions of a multicolor fluorescent cassette are crossed to the draculin:CreERT2 line to enable clonal labeling of lateral plate mesoderm lineages. By treating with 4-OHT at 24 hpf just after HSC specification, individual stem cell lineages express unique fluorescent hues which can be quantified in the adult marrow. (B) Families of Zebrabow-M;draculin:CreERT2 animals injected with either clodronate liposomes or the irf8 morpholino exhibit reduced numbers of HSC clones in the adult marrow, even when macrophages are not depleted until after emergence from the VDA. Mean +/− s.d., Unpaired t test; *P<0.05, ***P<0.001. (C) Macrophages (mpeg1:EGFP+) which have interacted with HSPCs (runx1+23:mCherry+) and removed fluorescent material can be harvested by FACS. (D) Macrophages which engage HSPCs are marked by lrp1ab and c1qa. Spectral scale reports z-scores.