Table 1. PCR primers for Plasmodium falciparum detection.
Gene | Primers | Sequence | band size (bp) |
---|---|---|---|
Primary PCR | |||
Mitochondrial coxI gene | rPLU5 | (5′-GAC CTG CAT GAA AGA TG-3′) | 1200 |
rPLU6 | (5′-GTA TCG CTT TAA TAG GCG-3′) | ||
Nested PCR | |||
18S rRNA | fal1 | (5’-GGAATGTTATTGCTAACAC-3’) | 205 |
fal2 | (5’-AAT GAA GAG CTG TGT ATC-3’) |