Fig 7A: In tab-1(ok2198) mutants, expression of both nlp-42(syb3238) and NeuroPAL reporters in AIN is lost. tab-1(ok2198) mutants also showed defects in unc-17(otIs576) reporter expression in AIN and AVD. No loss of reporter expression was observed in ttx-3(ot22) mutants. Representative images of wild type and mutant worms are shown with 10 μm scale bars. Fig 7B:
tab-1 is expressed in various neurons derived from the ABala lineage (adapted from Ma et al., 2021). In tab-1(ok2198) mutants, defects in NeuroPAL reporter expression, including ultrapanneuronal (UPN) reporter expression, are seen in neurons which express tab-1 embryonically. Representative images of wild type and mutant worms are shown with 10 μm scale bars. In all panels, neurons of interest are outlined in solid white when expressing wildtype reporter colors, and dashed white when one or all colors are lost. P-values were calculated by Fisher’s exact test.