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. 2022 Sep 30;18(9):e1010372. doi: 10.1371/journal.pgen.1010372

Fig 10. The OTX-type homeobox gene ttx-1 affects the differentiation of the ring interneurons RIP and RIB.

Fig 10

Fig 10A: ttx-1 locus showing different alleles used in this study. Fig 10B: The ttx-1(ot1264) cis-regulatory allele loses ttx-1(syb1679) expression in RIP, RIB and M2 neurons. Representative images of wild type and mutant worms at the L1 and adult stage are shown with 10 μm scale bars. Graphs compare expression in wild type and mutant worms with the number of animals examined listed at the bottom of the bar. P-values were calculated by Fisher’s exact test. Fig 10C: ttx-1 and unc-86 show synergistic effects in RIP differentiation. Representative images of nlp-73(syb4406) and nlp-51(syb3997) reporter allele expression in wild type and mutant worms are shown with 10 μm scale bars. Graphs compare expression in wild type and mutant worms with the number of neurons examined listed at the bottom of the bar. P-values were calculated by Fisher’s exact test. Fig 10D: The ttx-1(ot1264) cis-regulatory allele affects RIB differentiation. Representative images of sto-3 (otIs810) and unc-46 (otIs568) reporter transgene expression in wild type and mutant worms are shown with 10 μm scale bars. Graphs compare expression in wild type and mutant worms with the number of animals examined listed at the bottom of the bar. P-values were calculated by Fisher’s exact test.