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. 2022 Sep 30;18(9):e1010372. doi: 10.1371/journal.pgen.1010372

Fig 12. Derepression of dopaminergic terminal feature and dopaminergic regulatory signature in unc-86 mutants.

Fig 12

Fig 12A: unc-86(ot1158) and unc-86(n846) mutant animals ectopically express markers of ADE identity, including NeuroPAL (otIs669), reporter transgenes for genes involved in dopamine synthesis, including bas-1 (otIs226), cat-1 (otIs224), cat-2 (nIs118), cat-4(otIs225) and dat-1(vtIs1), and a flp-33 CRISPR reporter (syb3195). Representative images of wild type and mutant worms are shown with 10 μm scale bars. Graphs compare expression on the left side (ADEL) and the right side (ADER) in wild type and mutant worms with the number of animals examined listed at the bottom of the bar. Fig 12B: unc-86(ot1158) mutant animals show a derepression of CRISPR/Cas9-engineered reporter alleles of ceh-43(syb5073) and ast-1(vlc19) in cells of the anterior deirid lineage. Representative images of wild type and mutant worms are shown with 10 μm scale bars (non-neuronal expression depicted with an asterisk). Graphs compare expression on the left side (ADEL and AIZL) and the right side (ADER and AIZR) in wild type and mutant worms with the number of animals examined listed at the bottom of the bar. Fig 12C, FigD: ast-1 and ceh-43 are epistatic to unc-86. The derepression of expression of the cat-2 reporter transgene (otIs199) in unc-86(ot1158) mutant is suppressed in an ast-1(ot417) or an ast-1(ot406) mutant background. Note that both alleles are hypomorphic alleles (null alleles are lethal) [13,78]. Neurons of interest are outlined in solid white when expressing WT reporter colors, and dashed white when one or all colors are lost. Representative images of wild type and mutant worms are shown with 10 μm scale bars. Graphs compare expression on the left side (ADEL) and the right side (ADER) in wild type and mutant worms with the number of animals examined listed at the bottom of the bar. In all panels, neurons of interest are outlined in solid white when expressing wildtype reporter colors, and dashed white when one or all colors are lost and P-values were calculated by Fisher’s exact test.