Table 1. Comparing expression patterns of CRISPR/Cas9-engineered reporter alleles with fosmid-based reporter transgenes.
| Homeobox gene | Reporter allele 1 | CRISPR/Cas9-engineered reporter allele | Only observed with fosmid reporter 2, not with reporter allele | |
|---|---|---|---|---|
| also observed with fosmid reporter 2 | not observed with fosmid | |||
| vab-3 | devKi190 | ASK, BAG, OLQ, CEP, RIF, RIV, AS11, VA11, VD12 | ADA, OLL, URA, URB, URY | PVM*, SMB*, ALM, PLM, AVD, DBs, VBs, SAA |
| lim-7 | devKi125 | RIA, URY, OLL, PVN, PHC, AVB, AIB, AIM, BAG, M1, M5 | ALA, MI, AWC, I3, I4, BAG | AFD*, PVC*, RME |
| ceh-27 | syb2714 | I6, RIM, RIP, RME, RMF | AVL | none |
| ceh-43 | syb5073 | ADE, AIZ, ASJ, BDU, CAN, CEP, IL1, PDE, PVQ, SDQ | AIN | URB |
| ceh-45 | devKi191 | I1, MI | RIB | none |
| ceh-30 | syb4678 | SDQ | none | ubiquitous |
| ceh-31 | devKi250 | URA, URB, AVB, SDQ, PVR | none | none |
| ceh-16 | syb2880 | AIZ, RIF, RIG | none | none |
| ceh-36 | syb2934 | ASE, AWC, ASI, AWA | none | none |
| ceh-12 | devKi186 | VB | none | none |
| unc-30 | hzhCR1 | ASG, AVJ, DD, PVP, VD | none | none |
| ceh-17 | devKi180 | ALA, SIA | none | none |
| ceh-5 | devKi103 | embryo only | none | none |
| ceh-51 | devKi72 | embryo only | none | none |
| ceh-82 | devKi181 | none | none | ubiquitous |
| ceh-28 | devKi192 | M4 | none | none |
| ceh-2 | devKi17 | I3, M3 | none | NSM (dim) |
| ceh-10 | devKi101 | AIY, CAN, RID, DVC 3 | N/A 3 | N/A 3 |
Underlined neuron classed names indicate scRNA transcript found in this neuron in CeNGEN scRNA dataset [19]. When marked with *, transcript levels were very low, compared to other neurons that express the gene.
1 Generated by CRISPR/Cas9-based genome engineering. ot alleles were generated in our lab, syb alleles by Sunybiotech, dev and hzh alleles were kindly provided by Zhuo Du.
2 As described in [6]
3 the previously reported expression pattern [6] was not based on a fosmid but on a personal communication about another reporter allele.