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. 2022 Sep 30;18(9):e1010415. doi: 10.1371/journal.pcbi.1010415

Fig 6. An RNA Polymerase II dosage series.

Fig 6

Tunable, in vivo manipulation of RNA polymerase II activity using an rpb-2 auxin-inducible degron. A. The lifespan of rpb-2::AID;TIR1 populations exposed to a set of increasing concentrations of alpha-Naphthaleneacetic acid (NAA), shown as Kaplan-Meier survival curves. b. The time-dependent trajectory of the risk of death calculated for the same populations. c. At each dosage, the effect of NAA on the time of vigorous movement cessation and lifespan are compared. Error bars indicate 95% confidence intervals of AFT regression parameter estimates. Data was fit by a tri-phasic linear model (grey lines; statistical methods). Dotted line indicates the x = y unity line. d. The same analysis, but comparing vigorous movement cessation to remaining lifespan. e. Estimates of Rf and Δμ, quantifying the magnitude of disproportionate and proportionate effects of each NA concentration on VMC and lifespan (S5 text; Statistical methods). Error bars indicate 95% confidence intervals. Black lines indicate linear fits to each concentration range; < 187 μM and > 375 μM.