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. 2022 Sep 30;17(9):e0275520. doi: 10.1371/journal.pone.0275520

Table 4. The counts (log10 16S rDNA gene copies g-1 of colonic digest) of different bacterial groups measured by quantitative real-time polymerase chain reaction (qPCR).

Bacteria Dietary patterns ADHD scores
Healthy Processed food Fish and shellfish Meat p-value Control ADHD p-value
Total bacteria 11.40±0.311) 11.41±0.21 11.24±0.23 11.17±0.21 NS2) 11.37±0.14 10.99±0.24 NS4)
Bifidobacterium 10.14±0.13 10.35±0.21 10.11±0.14 10.25±0.13 NS 10.16±0.08 10.41±0.15 NS
Lactobacillus 8.87±0.17 8.59±0.28 8.67±0.19 8.49±0.26 NS 8.67±0.12 8.70±0.29 NS
Clostridium 7.73±0.20 7.57±0.33 7.38±0.31 7.64±0.26 NS 7.39±0.13 8.51±0.16* <0.055)
Enterobacter 6.57±0.15b 7.53±0.19a 7.18±0.37ab 7.20±0.26ab <0.053) 6.91±0.13 7.84±0.28* <0.05
Escherichia coli 6.21±0.16 6.95±0.36 6.75±0.33 6.84±0.23 NS 6.53±0.14 7.22±0.41 NS
Lactobacillus: Enterobacter 2.30±0.17a 1.06±0.19b 1.49±0.41ab 1.29±0.38ab <0.05 2.09±0.14 0.86±0.30* <0.05

1) Values represent the mean ± standard error of the mean (SEM)

2) NS, there were no significant differences by one-way analysis of variance (ANOVA) test

3) abc Groups with different letters in the same row are significantly different by one-way ANOVA with Duncan’s multiple range test

4) NS, there were no significant differences by Student’s t-test

5) The statistical significance between the control and ADHD groups was analyzed by the Student’s t-test (*p < 0.05)