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. 2001 Jun;183(12):3712–3720. doi: 10.1128/JB.183.12.3712-3720.2001

FIG. 2.

FIG. 2

β-Galactosidase activities. (A) β-Galactosidase activities driven by the rpoS-lacZ fusion pRPO220B. All measurements were done in triplicate; the mean and standard error are shown. Cells harboring plasmids were grown for 16 h in LB medium in the presence of appropriate antibiotics. P. putida MT17 is a psrA null mutant of parent strain WCS358, P. putida MT17R is also a psrA null mutant, and P. aeruginosa MKV8 is a psrA null mutant of parent strain PAO1. Plasmid pRPO220B (IncP; Tcr) is an rpoS-pacZ transcriptional fusion, whereas pMKP25 (IncQ; Cmr) harbors a 2.5-kb PstI insert of P. putida WCS358 DNA carrying the complete psrA gene. (B) β-Galactosidase activities driven by the psrA-lacZ fusion pPPSR220. Cells harboring plasmids were grown for 16 h in LB medium in the presence of appropriate antibiotics. See the text for details. Columns: a, P. putida WCS358(pRPO220B); b, P. putida MT17(pRPO220B); c, P. putida MT17(pRPO220B)(pMKP25); d, P. aeruginosa PAO1(pRPO220B); e, P. aeruginosa MKV8(pRPO220B); f, P. aeruginosa MKV8(pRPO220B)(pMKP25); g, P. putida MT17R(pRPO220B); h, P. putida MT17R(pRPO220B)(pMKP25); i, P. putida WCS358(pMP220); j, P. putida WCS358(pPPSR220); k, P. putida rpoS::Tn5 WCS358(pPPSR220); l, P. putida MT17(pPPSR220); m, P. putida MT17R(pPPSR220); n, P. putida WCS358(pMP220).