FIG. 3.

Gene map and protein sequence alignments. (A) Genetic organization of the region (PstI fragment; see text for details) containing the psrA regulatory gene in P. putida WCS358. Shown is the Tn5 insertion position located in mutant MT17. Also shown is the Tn5 insertion (shown as Tn5-R) located in the regenerated psrA mutant, designated MT17R. The DNA sequence of this 2.5-kb fragment can be found in GenBank accession number AJ293485. (B) (i) Protein sequence alignment, using the one-letter code, of the first 60 amino acids of PsrA, a TetR regulator of V. parahaemolyticus (V.para) (GenBank accession number Q56726), and IfeR of A. tumefaciens (GenBank accession number O68442). (ii) Protein sequence alignment, using the one-letter code, of the PsrA proteins from P. putida (PsrA) and P. aeruginosa (PsrA*) (this study). In bold are shown the amino acids which constitute the TetR family signature pattern (Prosite accession number PS01081). A star indicates conservation of identical amino acids, a colon indicates a conserved substitution, and a period indicates a semiconserved substitution.