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. 2001 Jun;183(12):3712–3720. doi: 10.1128/JB.183.12.3712-3720.2001

FIG. 4.

FIG. 4

β-Galactosidase activities and Western analyses. (A) Growth and β-galactosidase (β-Gal.) activities of P. putida WCS358(pRPO220B), psrA null mutant MT17(pRPO220B), and MT17(pRPO220B)(pMKP25). The values were determined with LB medium, and the means of triplicate experiments are shown. OD600, OD at 600 nm. (B) Cellular RpoS levels of overnight cultures (OD, 2.3) of P. putida WCS358 (lane 1), P. putida MT17 (lane 2), P. putida MT17(pMKP25) (lane 3), rpoS:: Tn5 P. putida (lane 4), purified His6-RpoS (lane 5), and prestained molecular mass markers (lane 6). Immunoblot analysis was performed with 30 μg of total cellular protein per lane, 20 ng of purified His6-RpoS (lane 5), and anti-RpoS antibodies. (C) Cellular PsrA levels of overnight cultures (OD, 2.4) of P. putida MT17 (lane 1), P. putida MT17(pMKP25) (lane 2), P. putida WCS358 (lane 3), and purified His6-PsrA (lane 4). Immunoblot analysis was performed with 30 μg of total cellular protein per lane, 20 ng of purified His6-PsrA (lane 4), prestained molecular mass markers (lane 5), and anti-PsrA antibodies.