TABLE 1.
Plasmids used
| Plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| pUC18 | Apr; ColE1 replicon | 47 |
| pBluescript KS | Apr; ColE1 replicon | Stratagene |
| pBluescript SK | Apr; ColE1 replicon | Stratagene |
| pQE30 | Apr; ColE1 replicon; His6 expression vector | Qiagen |
| pQE31 | Apr; ColE1 replicon; His6 expression vector | Qiagen |
| pREP-4 | lacI; Kmr; p15A replicon | Qiagen |
| pRK2013 | Kmr Tra+ Mob+; ColE1 replicon | 9 |
| pPH1JI | IncP1; Gmr | 1 |
| pMP220 | Promoter probe vector; IncP1; Tcr | 39 |
| pMP77 | Promoter probe vector; IncQ; Cmr | 39 |
| pLAFR3 | Broad-host-range cloning vector; IncP1; Tcr | 41 |
| pH3.5 | rpoS gene of strain WCS358 in pBluescript | 17 |
| pSB1075 | Apr; ColE1 replicon; AHL biosensor | 46 |
| pMK962 | rpoS promoter cloned in pCU18 | This study |
| pRPO77 | rpoS promoter cloned in pMP77 | This study |
| pRPO220A | rpoS incomplete promoter cloned in pMP220 | This study |
| pRPO220B | rpoS promoter cloned in pMP220 | This study |
| pPPSR18 | psrA promoter cloned in pUC18 | This study |
| pPPSR220 | psrA promoter cloned in pMP220 | This study |
| pCOS17 | pLAFR3 containing WCS358 DNA | This study |
| pCOS18 | pLAFR3 containing WCS358 DNA | This study |
| pAPC17 | pUC18 containing 680 bp of P. putida MT17 DNA bordering Tn5 | This study |
| pBSH10 | pBluescript containing 10-kb HindIII fragment from pCOS17 | This study |
| pBS25 | pBluescript containing 2.5-kb PstI fragment from pCOS17 | This study |
| pLM17E | pLAFR3 containing 5-kb EcoRI fragment from pCOS17 | This study |
| pLM17P | pLAFR3 containing 2.5-kb PstI fragment from pCOS17 | This study |
| pMKP25 | pMP77 containing 2.5-kb PstI fragment from pCOS17 | This study |
| pPSRPAO1 | 2.2-kb PCR EcoRI fragment from PAO1 genome harboring psrA in pBluescript | This study |
| pPSRPAO1::Tn5 | pPSRPAO1 with Tn5 insertion in psrA | This study |
| pSRE8 | pLAFR3 harboring 8-kb EcoRI fragment from pPSRPAO1::Tn5 | This study |
| pBS25::Tn5R | pBS25 with Tn5 insertion in psrA | This study |
| pCOS17::Tn5R | pCOS17 with Tn5 insertion in psrA | This study |
| pQEPSRA | psrA cloned in pQE30 | This study |
| pQERPOS | rpoS cloned in pQE31 | This study |
a
Apr, Kmr, Smr, Tcr, Gmr, and Cmr, resistant to ampicillin, kanamycin, streptomycin, tetracycline, gentamicin, and chloramphenicol, respectively.