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. 2022 Sep 30;8(39):eabf4792. doi: 10.1126/sciadv.abf4792

Fig. 2. Growth of Synechococcus and heterotrophic bacteria within the coculture system.

Fig. 2.

(A) Flow cytometric measurement showing Synechococcus and heterotrophic bacterial cell abundance over the 450 days of coculture period. Cocultures I and II are replicate culture systems. Each data point is an average of two replicates. The vertical dotted line approximately demarcates the start of each of the observed phases (P-I to P-VI). (B) Chlorophyll-a fluorescence, as an indicator of Synechococcus growth, was measured at 440/680 nm. Each data point is an average of three replicates. (C) Histogram showing the intensity of chlorophyll-a of the two subpopulations (S-I and S-II) related to Synechococcus sp. PCC7002. The error bars in each plot indicate the standard error of the mean. CC-I Syn., Synechococcus sp. PCC7002 abundance in coculture-I; CC-II Syn., Synechococcus sp. PCC7002 abundance in coculture II; Ax. Syn., Synechococcus sp. PCC7002 abundance in axenic culture; CC-I Bact., heterotrophic bacterial abundance in coculture I; CC-II Bact., heterotrophic bacterial abundance in coculture II.