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. 2022 Sep 30;8(39):eabo0514. doi: 10.1126/sciadv.abo0514

Fig. 4. Characterization of CAR T cells at single-cell level.

Fig. 4.

CAR+ (BFP+) cells from three independent CAR T cell productions were assayed by scRNA-seq. (A) An overview of the 28,117 cells that passed quality control and filtering for subsequent analyses in this study. Uniform Manifold Approximation and Projection (UMAP) plot showing the 15 clusters that were analyzed. (B) Heatmap showing signature genes of each cluster and putative assignments to cell types according to canonical marker genes. (C) UMAP plot overlaid with mRNA expression of T cell markers (CD4 and CD8A), memory markers (TCF7 and CCR7), activation markers (HLA-DRA and GATA3), effector enzymes (GZMA and PRF1), and exhaustion markers (LAG3 and TIGIT).