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. 2001 Jun;183(12):3729–3736. doi: 10.1128/JB.183.12.3729-3736.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 5 — Southern hybridization of various chloramphenicol-resistant Arthrobacter sp. mutants generated with pKGT452Cβ. Total DNA was digested with SphI (A) or BglII (B) and probed with the internal 1-kb SstI fragment of tnpA. As no SphI and BglII site occurs in the tnpA gene, one signal with a minimal size of 3.4 or 1.4 kb (SphI/BglII) is expected after insertion of Tn1409Cβ. Lane 1, digoxigenin-labeled λEcoRI/HindIII; lane 2 Arthrobacter sp. strain SU; lanes 3 to 23, Arthrobacter sp. strain SUX mutants D1 to D21; lane 24, Arthrobacter sp. strain SUX; lane 25, digoxigenin-labeled λEcoRI/HindIII. Mutants D4 and D5 (lanes 6 and 7) seem to be identical. All other mutants display different hybridization patterns, indicating a random insertion.