TABLE 2.
Comparison of IS1409 with some insertion sequences of the ISL3 family
IS element | Origin | Accession no. | Length of TnpA (aa) | Identity/similarity (%) | Length of IRc (bp) | Target duplication (bp) | Reference |
---|---|---|---|---|---|---|---|
IS1409 | Arthrobacter sp. strain TM1 | AF042490 | 435 | 25 | 8 | This work | |
IS1096a | Mycobacterium smegmatis | M76495 | 413 | 77/87 | 25 | 8 | 3 |
ISB11 | Brevibacterium linens | AF052055 | 436 | 69/79 | 25 | 8 | Unpublished data |
IS1411 | Pseudomonas putida | M57500 | 433 | 60/76 | 24 | 8 | 12 |
IS13869 | Brevibacterium lactofermentum | Z66534 | 436 | 42/57 | 26 | 8 | 4 |
IS31831 | Corynebacterium glutamicum | D17429 | 436 | 42/56 | 24 | 8 | 36 |
IS2001 | Bifidobacterium lactis | AJ243948 | 442 | 43/55 | 26 | ?d | Unpublished data |
IS204 | Nocardia asteroides | U10634 | 377b | 30/47 | 23 | 8 | 43 |
ISPs1 | Pseudomonas stutzeri | AJ012352 | 425 | 27/48 | 24 | 8 | 2 |
ISAe1 | Alcaligenes eutrophus | M86608 | 408 | 27/41 | 24 | 8 | 15 |
IS1165 | Leuconosioc mesenteroides | X62617 | 412 | 23/38 | 24 | 8 or 3 | 11 |
IS1001 | Bordetella parapertussis | X66858 | 406 | 22/37 | 28 | 6 or 8 | 34 |
ISL3 | Lactobacillus delbrueckii | X79114 | 434 | 20/37 | 38 | 8 | 8 |
IS1181 | Staphylococcus aureus | L14544 | 439 | 19/38 | 23 | 8 | 6 |
IS1096 is the only one of this group of IS elements which additionally contains a putative resolvase gene
IS204 may contain a frameshift sequencing error or represent a nonfunctional IS element, as the conserved C-terminal domain of the transposase displayed in Fig. 1 occurs in a different reading frame from the tnpA gene.
IR, inverted repeat.
?, data not available.