Figure 1.

R-Cu treatment up-regulates anti-oxidant enzymes in OSCC tumour cells. FFPE sections of tumour tissues were stained with antibodies against superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx), and examined under fluorescence microscopy. Upper panels provide representative images (scale bar 10µm); lower panels represent quantitative analysis of cells positive for respective anti-oxidant enzymes. One thousand cells were analysed in each case and the number of cells showing positive signals were recorded. Combined statistical comparison of pre versus post treatment values of all four treatment groups with the control group was done using two-way ANOVA; p values of which are given under each graph. Comparison of pre versus post treatment values within each treatment group was performed by paired Student’s t-test. Results of the latter are represented as mean ± SEM of 5 patients in each treatment group. *< p < 0.05; **< p < 0.01; ***< p < 0.001; ns, not significant.