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. 2022 Aug 4;6(10):1458–1470. doi: 10.1038/s41559-022-01838-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — Comparison of PacBio and Illumina sequencing. PacBio amplicons were compared with metagenomes (mTags), V4 amplicons, and V9 amplicons from three marine samples corresponding to the pico size fraction from the Malaspina expedition²⁷. Station 76|Surface did not have V9 amplicon data. ASVs = Amplicon Sequence Variants. (a) Number of reads and ASVs for each sample for each marker. The mTags represent sequence length of ca. 100 bp, so no ASV level is available, as this short length does not give enough resolution. More PacBio sequences were generated for each sample compared to Illumina sequences. (b) Comparison of PacBio ASVs (that is de-noised, preclustered sequences) with the ones given by V4 amplicons. A similar comparison with V9 ASVs was not carried out as not all samples had V9 Illumina data available. Around half of the sequences were shared, which represented the majority of reads.