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. 2022 Sep 30;12:16459. doi: 10.1038/s41598-022-20816-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Augmentation of in-vivo video data with HSI. (a) Start frame from the color sensor acquired during the HSI record. The blue outline indicates the region visible in HSI. (b) An exemplary target frame of the video, 20 s after HSI was performed. (c) Pseudocolor image reconstructed from hyperspectral data. (d) Static color map representing the tissue oxygenation according to the color scale. (e) Semitransparent overlay of the oxygenation map and the grayscale of the target frame after registration and postprocessing.