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. 2022 Apr 5;29(10):1982–1995. doi: 10.1038/s41418-022-00990-5

Fig. 4. PRMT4 promotes DOX-induced ferroptosis in vitro.

Fig. 4

NRVMs were infected with adenovirus (Ad-NC, Ad-PRMT4, Ad-shNC, or Ad-shPRMT4) and treated with RSL3 (0.5 μM) or DOX (2 μM) for 24 h. a, b Quantitative analysis of cell viability, *P < 0.001, #P < 0.05 (a). *P < 0.001, #P < 0.01 (b); c, d Cellular lipid ROS was determined, *P < 0.01, #P < 0.05 (c). *P < 0.01, #P < 0.05 (d); e, f Representative western blots of PRMT4, NCOA4, and GPX4 were shown; g, j Quantitative analysis of the protein level of PRMT4, *P < 0.05, #P < 0.001, $P < 0.05 (g). *P < 0.001, #P < 0.001, $P < 0.01 (j); h, k Quantitative analysis of the protein level of GPX4, *P < 0.05, #P < 0.05 (h). *P < 0.01, #P < 0.05 (k); i, l Quantitative analysis of the protein level of NCOA4, *P < 0.001, #P < 0.001 (i). *P < 0.001, #P < 0.01 (l).