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. 2022 Feb 25;43(10):2624–2635. doi: 10.1038/s41401-022-00885-8

Fig. 3. Dapa inhibits MAPK signaling and inflammation in cardiomyocytes.

Fig. 3

H9c2 cells were pretreated with Dapa, and were then stimulated with PA for 1 h. a H9c2 cells were fixed and stained with anti-p-cJUN antibody, and counterstained with DAPI. Representative images were shown (scale bar = 25 μm). b Cell lysates were collected and proteins of p-ERK, p-JNK and p-p38 were detected with total proteins as loading control. c Cells were pretreated with Dapa, and were then stimulated with PA for 8 h. Total mRNA was isolated using TRIzol, and mRNA levels of pro-inflammatory genes were detected with β-actin as the normalization control. n = 3; Means ± SEM; One-way ANOVA followed by Turkey post-hoc tests; *P < 0.05, compared with Ctrl group, #P < 0.05, compared with PA group.