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. 2022 Feb 25;43(10):2624–2635. doi: 10.1038/s41401-022-00885-8

Fig. 6. Dapa attenuates MAPK/AP1 cascades and cardiac inflammation in HFD mice.

Fig. 6

Mice were randomly divided into 3 groups, including LFD, HFD and HFD + Dapa. After 3 months of high fat diet, mice were treated with 1 mg·kg−1·d−1 Dapa for another 2 months. a Western blot analysis of proteins in MAPK pathway in heart tissue. GAPDH was used as loading control. b Representative images of anti-p-cJUN immunofluorescence staining in heart sections (scale bar = 25 μm). c Quantitative analysis of inflammatory genes in heart tissue, and β-actin was used as normalization control. d ELISA assay of inflammatory cytokines in heart tissues. e, f Representative images of anti-TNF-α and anti-CD68 immunochemistry staining in heart sections (scale bar = 30 μm). n = 8; Means ± SEM; One-way ANOVA followed by Turkey post-hoc tests; *P < 0.05, compared with LFD group, #P < 0.05, compared with HFD group.