Figure 3.

pH and thermal stability of phi50-12. (a) 10⁶ PFU of phi50-12 were subjected to various pH at 30 °C for one hour and titrated by plaque assay using a double-layer method; (b) phi50-12 stability (10⁶ PFU) under various temperatures for 1 h was measured by plaque assay. The survival rate was measured by comparison with initial phage number. The experiments were performed in triplicates and the data are shown as the mean ± SEM. Student’s t test was performed for significance. Ns no significance; **p < 0.01; ***p < 0.001.