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. 2022 Mar 15;43(10):2636–2650. doi: 10.1038/s41401-022-00889-4

Fig. 6. Sotagliflozin enhances vascular endothelial cells migration and proliferation potentials under hyperglycemia through skeletal muscle cells’ secretome.

Fig. 6

a, b Proliferation potential of HUVECs cultured with CM-HG + Sota, as examined using EdU incorporation assay. Representative images (a; scale bars: 100 μm) and quantification results (b) were shown. c, d Migration potential of HUVECs cultured with CM-HG + Sota, as analyzed using transwell migration assay. Representative images (c; scale bars: 200 μm) and quantification results (d) were shown. e, f F-Actin polymerization in HUVECs cultured with CM-HG + Sota, as examined using phalloidin staining. Representative images (e; scale bars: 100 μm for upper panels and 50 μm for lower panels) and quantification of fractal dimension (f) were shown. Data were presented as mean ± SD (n = 6). CM-NG conditioned media obtained from 10% DMSO-treated C2C12 cells under normoglycemia, CM-HG conditioned media obtained from 10% DMSO-treated C2C12 cells under hyperglycemia, CM-HG + Sota conditioned media obtained from 10 μM sotagliflozin-treated C2C12 cells under hyperglycemia; ***P < 0.001.