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. 2022 Mar 16;43(10):2687–2695. doi: 10.1038/s41401-022-00890-x

Fig. 4. ENKUR binds to β-catenin to decrease MYH9 expression.

Fig. 4

a qPCR analysis of MYH9 expression in ENKUR-overexpressing LUAD cells and control cells. b The expression levels of β-catenin, c-Jun and MYH9 in ENKUR-overexpressing LUAD cells and control cells. c Western blot analysis was used to determine the effects of β-catenin overexpression on the expression of β-catenin, c-Jun and MYH9 in ENKUR-overexpressing LUAD cells. Co-IP (d) and immunofluorescence assay (e) were used to detect the interaction between ENKUR and β-catenin in ENKUR-overexpressing A549 cells. f Co-IP in A549 cells transfected with plasmids containing different domains of ENKUR was utilised to determine the domain that binds to β-catenin.