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. 2022 Mar 16;43(10):2687–2695. doi: 10.1038/s41401-022-00890-x

Fig. 5. ENKUR attenuated MYH9/USP7-mediated c-Myc deubiquitination.

Fig. 5

a Western blot analysis was used to detect the effects of ENKUR and MYH9 on c-Myc protein expression. b The effects of ENKUR and MYH9 on the stability of c-Myc are shown in A549 cells incubated with cycloheximide at different time points with or without MG132 treatment. c Co-IP assay of the interactions between MYH9, USP7, c-Myc and ubiquitin in A549 cells. d and e A Co-IP assay was used to detect ubiquitinated c-Myc in MYH9-silenced A549 cells, USP7-silenced A549 cells and the corresponding control cells after treatment with MG132 for 6 h. f Western blot analysis was conducted to assess the effect of USP7 depletion on c-Myc expression in LUAD cells transfected with the MYH9 plasmid. g The expression of c-Myc in the cytoplasm and nucleus of LUAD cells transfected with the ENKUR or ENKUR + MYH9 plasmid.