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a The binding sites of c-Jun in the ENKUR promoter were predicted by JASPAR. The mRNA (b) and protein (c) levels of ENKUR were determined in c-Jun-overexpressing LUAD cells and control cells. qPCR assays (d) and gel electrophoresis (e) after ChIP were used to detect the binding of c-Jun to the ENKUR promoter in A549 and SPC-A1 cells. f The levels of p-PI3K, PI3K, p-AKT, AKT, c-Jun and ENKUR in LUAD cells treated with CB.
