TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Azoarcus sp. strain BH72 | Wild type | 45 |
| BHΔfdxN | fdxN in-frame deletion in BH72 | This study |
| BHΔfdxN(pfdxN) | BHΔfdxN complemented with pfdxN | This study |
| E. coli DH5αF′ | F′ endA1 hsdR17(rK− mK+) supE44 thi-1 recA1 gyrA relA1 φ80lacZΔM15 Δ(lacZYA-argF)U169 | 13 |
| Plasmids | ||
| pUC19/18 | Apr | 59 |
| pBSKII/pBKSII | Apr | Stratagene |
| pLAFR3 | Tetr, cosmid vector | 56 |
| pEN9 | Apr, KpnI fragment from GenBank clone pBGVN9 carrying nif gene cluster in pUC19 | This study |
| pEN94 | Apr, subclone from pEN9 | This study |
| pEN9ΔF | Apr, fdxN deletion on pEN9 | This study |
| pfdxN | Tetr, fdxN gene fused to the nifH promoter region on pLAFR3 | This study |