TABLE 2.

Primers used

Application	Primer and sequence (position)a
nifK probe	—b
ORF1 probe	TE27: 5′CCTGCGCTTCACCTATTGTT3′ (6240–6259)
	TE28: 5′AGCGAAATCCAACTCCGCCA3′ (110 nt downstream of ORF1)
fdxN probe	TE29: 5′ACCAAGTCGATCACCGACAA3′ (5200–5219)
	TE30: 5′CTACAAAAAACAGGGAGCC3′ (5384–5366)
Test for fdxN deletion	TE14: 5′CCGCCAGTGGTGGGCTTTCG3′ (4911–4930)
	TE19: 5′GACGACCTCGACACCGGGCA3′ (5499–5518)
Amplification of nifHDK promoter region	fdxpro2(PstI): 5′AACTGCAGAGTCGAACAGCCAGCCGCGCA3′ (34–54)
	fdxprorev(BamHI): 5′AAGGATCCAAGAAAAAAACTACCGGGTGTCCTGGG3′ (600–574)
fdxN gene	fdxvor(BamHI): 5′CGGGATCCGGAGAAGGAATCATGGCTCTG3′ (5125–5145)
	TE36(EcoRI): 5′ACGAGCCTCATGGGAATTC3′ (5421–5403)
RT-PCR	nifKfw: 5′CGCCTTCGCGGCACCCGT3′ (4799–4816)
	Fdxrev: 5′CAGTCACCACAGGACGTGCAT3′ (5183–5163)
Primer extension	TH25rCy: 5′TGCCGATACCGCCCTTGCC (668–650)

^aNucleotide numbering according to GenBank accession number AF200742. Underlined sequences are restriction sites. 

^bSee reference 20.