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. 2022 Sep 30;12:16415. doi: 10.1038/s41598-022-20186-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of Jurkat cells exosomes purification. Jurkat cells exosomes were purified from two fractions. The two fractions were quantified using the Nano- Sight tracking analysis (NTA). Represented images of the quantification of the first fraction (using dilution factor 1:200) (A) and the quantification of the second fraction (using dilution factor 1:6600) (B) are shown. (C) Quantification of total protein concentration of the two fractions of purified Jurkat cells exosomes. The graphs values represent mean concentration ± S.E of three independent experiments. *Indicates PV < 0.001. D. Examples of single exosomes images captured by EM.