Table 2.
Setup for immunohistochemistry and intravital imaging
| Antibodies used for immunostaining | ||||
|---|---|---|---|---|
| Target | Primary antibodies | Secondary antibodies | ||
| Antibody | Dilution | Antibody | Dilution | |
| Albumin | Anti-albumin, rabbit | 1:500 | Ultra-Map anti-rabbit HRP |
Automatic Discovery Ready to use |
| Leukocytes | Anti-mouse CD45, rat | 1:400 | Ultra-Map anti-rat HRP | |
| Inflammatory subpopulation of macrophages | Anti-S100A4, rabbit | 1:200 | Ultra-Map anti-rabbit HRP | |
| Intravital imaging conditions of ochratoxin A and TMRE | ||||
|---|---|---|---|---|
| Dye/toxin | Marker for | Dose [mg/kg] | Vehicle | Excitation range [nm] |
| TMRE | Mitochondrial membrane potential | 0.96 | Methanol/PBS (1:1) | 780–820 |
| Ochratoxin A | Ochratoxin A | 5 | 0.1 M NaHCO3 | 740–780 |