Fig. 6.

NFT pretreatment protects from various insults. HepG2 cells were pretreated with NFT (40 μM) for 48 h, followed by a medium change and maintenance in the absence of NFT for an additional 48 h. Then, a NFT or b the redox cycler, paraquat, were added at a toxic concentration range for 48 h. Cell viability was analyzed by reduction of resazurin and by the LDH release assays, and cell homogenates were analyzed for their glutathione content. Data are means of 3 independent experiments ± SD, individual data are illustrated by dots. c Primary human hepatocytes (PHH, 150,000 cells/cm²) from three individual donors (HUM4229, HUM4108, HUM181501B) were maintained in the presence of NFT (25 μM) for 48 h, followed by a period of 48 h in the absence of NFT. Then, rotenone (5 μM), paraquat (500 μM), or a damaging concentration of NFT (125 μM) were added for 24 h. Viability was assessed by the resazurin reduction assay, cell homogenates were assessed for their glutathione content. Differences were tested for significance by two-way ANOVA (pretreatment vs. w/o pretreatment for individual NFT or paraquat concentrations), followed by a Bonferroni’s post hoc test *p < 0.05