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. 2022 Aug 31;59(11):6817–6833. doi: 10.1007/s12035-022-02995-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Expression of Npn-2 in adult rat hippocampus. A, B The mRNA levels of Npn-2 and its ligand Sema3F in rat hippocampus at different developmental stages (P0, P7, P14, P28, P42, and P70) were measured by real-time quantitative PCR. Npn-2 and Sema3F mRNA maintained their expression in adult hippocampus. C, D The protein levels of Sema3F and Npn-2 in rat hippocampus at different developmental stages (P0, P7, P14, P28, P42, and P70) were assessed by western blot analysis. Npn-2 and Sema3F retained their expression in adult hippocampus. E, F Quantitation of C and D, respectively. G Immunofluorescent detection of Npn-2 expression in rat hippocampus. Immunofluorescent staining with Npn-2 (green) and DAPI (blue) showed that Npn-2 is expressed in adult hippocampus, mainly in the neuropil, dentate gyrus granule cell inner molecular layer, and mossy fibers. Scale bar, 100 μm