Table 2.
Effect of inducer (isobutyronitrile) concentration on the production of nitrilase by G. terrae at different incubation time periods
| Inducer concentration (%, v/v) at 0, 16 and 24 h | Nitrilase activity (U/mg dcw) at different incubation time | |||
|---|---|---|---|---|
| 32 h | 40 h | 48 h | 56 h | |
| 0.1 | 1.4 | 2.6 | 1.42 | 0.34 |
| 0.2 | 1.54 | 2.9 | 1.5 | 0.44 |
| 0.3 | 1.62 | 3.2 | 1.76 | 0.52 |
| 0.4 | 2.2 | 3.9 | 1.8 | 0.69 |
| 0.5 | 2.5 | 4.5 | 1.94 | 0.5 |
| 0.6 | 2.1 | 2.95 | 1.6 | 0.42 |
| 0.7 | 1.5 | 2.1 | 1.68 | 0.38 |
| 0.8 | 1.2 | 2.8 | 1.32 | 0.25 |
| 0.9 | 0.5 | 0.8 | 0.1 | 0.04 |
| 1.0 | 0.02 | 0.03 | 0.02 | 0.01 |
Bold values represent the maximum nitrilase activity at different incubation time with 0.5% inducer concentration