Table 2.
Genetic variability at 12 microsatellite loci in four L. fimbriatus populations
Microsatellite Locus | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Population | LF-01 | LF-03 | LF-04 | LF-07 | LF-08 | LF -09 | LF -10 | LF -11 | LF -12 | LF -13 | LF -15 | LF-16 | Average across loci |
Mahanadi | |||||||||||||
NA | 8 | 15 | 5 | 12 | 8 | 12 | 8 | 6 | 19 | 14 | 12 | 7 | 12 |
HO | 0.704 | 0.931 | 0.772 | 1.00 | 1.000 | 0.976 | 0.825 | 0.553 | 0.883 | 1.000 | 0.733 | 1.000 | 0.865 |
HE | 0.623 | 0.863 | 0.571 | 0.887 | 0.705 | 0.751 | 0.675 | 0.560 | 0.909 | 0.892 | 0.825 | 0.775 | 0.753 |
P | 0.149 | 0.195 | 0.147 | 0.007 | 0.128 | 0.042 | 0.037 | 0.010 | 0.036 | 0.00* | 0.191 | 0.032 | |
1 − HO/HE | 0.132 | − 0.07 | − 0.35 | − 0.12 | − 0.42 | − 0.30 | − 0.22 | 0.01 | 0.02 | − 0.12 | 0.11 | − 0.29 | − 0.15 |
Godavari | |||||||||||||
NA | 10 | 10 | 11 | 4 | 6 | 9 | 1 | 9 | 15 | 6 | 12 | 9 | 15 |
HO | 1.00 | 1.00 | 1.00 | 1.000 | 1.000 | 1.000 | 0.00 | 1.000 | 0.978 | 0.765 | 1.000 | 1.000 | 0.895 |
HE | 0.853 | 0.864 | 0.846 | 0.673 | 0.698 | 0.846 | 0.00 | 0.767 | 0.853 | 0.628 | 0.865 | 0.805 | 0.725 |
P | 0.002 | 0.549 | 0.408 | 0.000 | 0.004 | 0.113 | 1.000 | 0.081 | 0.035 | 0.505 | 0.067 | 0.010 | |
1 − HO/HE | 0.174 | − 0.15 | − 0.18 | − 0.49 | − 0.43 | − 0.18 | 0.00 | − 0.30 | − 0.14 | − 0.22 | − 0.15 | − 0.24 | − 0.23 |
Krishna | |||||||||||||
NA | 7 | 6 | 7 | 4 | 5 | 5 | 4 | 4 | 6 | 5 | 12 | 5 | 6.82 |
HO | 0.736 | 0.609 | 0.526 | 0.428 | 0.454 | 0.760 | 0.777 | 0.590 | 0.595 | 0.446 | 0.869 | 0.708 | 0.657 |
HE | 0.802 | 0.797 | 0.785 | 0.657 | 0.696 | 0.738 | 0.594 | 0.590 | 0.825 | 0.699 | 0.801 | 0.687 | 0.729 |
P | 0.105 | 0.093 | 0.043 | 0.001 | 0.035 | 0.030 | 0.003 | 0.661 | 0.563 | 0000* | 0.003 | 0.134 | |
1 − HO/HE | 0.083 | − 0.23 | 0.33 | 0.350 | 0.349 | − 0.03 | − 0.31 | 0.00 | 0.28 | 0.36 | − 0.08 | − 0.03 | 0.099 |
Kaveri | |||||||||||||
NA | 6 | 6 | 5 | 3 | 8 | 7 | 1 | 7 | 10 | 6 | 7 | 7 | 14.37 |
HO | 1.00 | 0.230 | 1.00 | 1.000 | 1.000 | 1.000 | 0.000 | 1.000 | 1.000 | 0.975 | 1.000 | 0.708 | 0.914 |
HE | 0.821 | 0.756 | 0.771 | 0.669 | 0.823 | 0.814 | 0.000 | 0.825 | 0.847 | 0.697 | 0.750 | 0.687 | 0.705 |
P | 0.011 | 0.011 | 0.001 | 0.000 | 0.058 | 0.014 | 1.000 | 0.113 | 0.001 | 0.096 | 0.013 | 0.035 | |
1 − HO/HE | − 0.22 | 0.178 | − 0.29 | − 0.50 | − 0.21 | − 0.23 | 0.000 | − 0.21 | − 0.18 | − 0.40 | − 0.33 | − 0.03 | -0.30 |
The data presented as no. of alleles (NA), expected heterozygosity (HE), observed heterozygosity (Ho), the p value for testing Hardy–Weinberg equilibrium, and fixation index (FST) = 1 − HO/HE in four populations of L. fimbriatus
NA number of allele, HO and HE observed and expected heterozygosity, f inbreeding co-efficient
*Significant deviation from HWE after Bonferroni correction (p < 0.0001)