Table 2.
Estimation of the main effects of the Central Composite Rotational Design (CCRD) planning for the total phenolics responses, hydrolytic activity (Ah), transfructosylation activity (At) and transfructosylation and hydrolytic activity ratio (At/Ah)
| Factors | Phenolics (mg GAE/100 g) | Ah (U/mL) | At (U/mL) | At/Ah | ||||
|---|---|---|---|---|---|---|---|---|
| F test | p-value** | F test | p-value* | F test | p-value** | F test | p-value* | |
| Model | 136.78067 | < 0.00001 | 5.680248 | 0.006568 | 14.05529 | 0.0001194 | 14.198570 | 0.000158 |
| Linear | ||||||||
| Sucrose (X1) | ns | ns | 3.54194 | 0.086544 | ns | ns | ns | ns |
| Yeast extract (X2) | 287.95244 | 0.000446 | 13.06001 | 0.004070 | 5.59533 | 0.034226 | 3.72814 | 0.074009 |
| Residue (X3) | 1980.55414 | 0.000025 | ns | ns | ns | ns | ns | ns |
| Quadratic | ||||||||
| Sucrose (X12) | ns | ns | 3.64123 | 0.082793 | 24.93996 | 0.000246 | 30.60593 | < 0.0001 |
| Yeast extract (X22) | ns | ns | 9.17586 | 0.011466 | ns | ns | 14.35550 | 0.001994 |
| Residue (X32) | 376.91877 | 0.000299 | ns | ns | ns | ns | ns | ns |
| Interaction | ||||||||
| 1 by 2 (X1X2) | ns | ns | 3.29664 | 0.096750 | ns | ns | ns | ns |
| 1 by 3 (X1X3) | ns | ns | 3.03333 | 0.109425 | 18.72025 | 0.000822 | ns | ns |
| 2 by 3 (X2X3) | ns | ns | ns | ns | 6.96561 | 0.020425 | ns | ns |
| Lack of fit | 7.932391463 | 0.057306784 | 1729.35843 | < 0.0001 | 68.663114 | 0.0025621 | 28.514979 | 0.0093004 |
| R2 | 0.96701 | 0.756 | 0.8122 | 0.7526 | ||||
**95% confidence level; * 90% confidence level
ns not significant