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. 2022 Mar 8;43(10):2573–2584. doi: 10.1038/s41401-022-00875-w

Fig. 1. ELA-21 alleviated VSMC inflammation and oxidative stress of SHRs.

Fig. 1

a ELA expression in VSMCs of WKY and SHR determined by semi-quantitative PCR. b, c inflammatory cytokines mRNA and protein level in SHR-VSMCs. d DHE fluorescence staining for detecting reactive oxygen species (ROS) in SHR-VSMCs. e NOX1, NOX4 mRNA and protein level in SHR-VSMCs. f Nuclear Nrf2 expression determined by immunofluorescent staining in SHR-VSMCs. g Nrf2 downstream protein (HO-1, NQO-1, GCLC and GCLM) expression in SHR-VSMCs. The measurements were made 24 h after ELA-21 (200 nM) was added to the medium of SHR-VSMCs. n = 4 per group in a–c, e and g; n = 6 per group in d and f. Values are mean ± SEM. *P < 0.05.