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. 2022 Mar 8;43(10):2573–2584. doi: 10.1038/s41401-022-00875-w

Fig. 2. ELA-21 alleviated Ang II-induced VSMC inflammation and oxidative stress of WKYs.

Fig. 2

a Inflammatory cytokines mRNA and protein level in WKY-VSMCs. b DHE fluorescence staining for detecting reactive oxygen species (ROS) in WKY-VSMCs. c, d NOX1, NOX4 mRNA and protein level in WKY-VSMCs. e Nuclear Nrf2 expression determined by immunofluorescent staining in WKY-VSMCs. f Nrf2 downstream protein (HO-1, NQO-1, GCLC and GCLM) expression. The cells were treated with ELA-21 (200 nM) for 2 h followed by Ang II (100 nM) for 24 h in WKY-VSMCs. n = 4 per group in a, c, d and f; n = 6 per group in b and e. Values are mean ± SEM. *P < 0.05.