Skip to main content
. 2022 Mar 8;43(10):2573–2584. doi: 10.1038/s41401-022-00875-w

Fig. 7. PI3K/Akt pathway mediated the effects of ELA-21 on Ang II-induced VSMC proliferation of WKYs.

Fig. 7

a, b Phosphoinositide3-kinase (PI3K) p85α and Akt phosphorylation in WKY-VSMCs; VSMC proliferation were evaluated with CCK-8 kits (c), PCNA expression (d) and EdU-positive cells (e). The PI3K/Akt inhibitor LY294002 (10 μM) and activator 740-YP (10 μM) were added into the medium 0.5 h before administration of ELA-21 (200 nM). The cells were treated with ELA-21 for 2 h followed by Ang II (100 nM) for 24 h in WKY-VSMCs. n = 4 per group in a, b and d; n = 6 per group in c and e. Values are mean ± SEM. *P < 0.05.