Skip to main content
. 2022 May 14;29(10):2009–2023. doi: 10.1038/s41418-022-00992-3

Fig. 2. Ezh2 enhances inflammasome activation in microglia.

Fig. 2

A ELISA of IL-1β in culture supernatants from WT and Ezh2-KO primary microglia that were primed with LPS for 4 h, or stimulated with nigericin or poly(dA:dT) for 1 h after LPS priming. Immunoblot analysis of caspase-1 processing in the supernatant (Sup) and the whole cell lysates (Wcl) from WT and Ezh2-KO microglia that were primed with LPS for 4 h or stimulated with nigericin (B) or poly(dA:dT) (C) for 1 h after LPS priming. D Confocal microscopic images (left) and quantification analysis (right) of NLRP3 (green) inflammasome assembly in Iba1+ microglia (red) of striatum from WT and Ezh2-MKO mice after unilateral intra-striatal perfusion with 6-OHDA for 3 days. Scale bar, 50 μm and 10 μm (n = 7). E Immunohistochemical images (left) and quantification analysis (right) of substantia nigra from WT and Ezh2-MKO mice after intra-striatal perfusion with PBS or 6-OHDA for 2 weeks. The splices were stained with anti-TH to mark neurons. Scale bar, 250μm, (WT, n = 10; Ezh2-MKO, n = 8).