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. 2022 Sep 30;12:16396. doi: 10.1038/s41598-022-20657-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of lipid peroxidation levels in the bro-ALI and alv-ALI models due to sham exposure and HTP-smoke exposure. (a, b) Colorimetric malondialdehyde (MDA) assay and (c, d) BODIPY 581/591 C11 (Lipid Peroxidation Sensor) assay using ferrostatin-1 by flow cytometry. Data are shown as medians and interquartile ranges. n = 6 per exposure condition; non-parametric statistical analysis (Wilcoxon signed rank test or Friedman test followed by Wilcoxon signed rank test, as appropriate); ns: not significant; ^#,¤,$,*p < 0.05. MDA levels were below the limit of detection (LOD) in both bro-ALI and alv-ALI sham samples. Therefore, a value just below the LOD (0.85 nM) of MDA is assigned to all the sham samples for statistical analysis. ALI: air–liquid interface; alv-ALI: alveolar mucosa model at ALI; bro-ALI: bronchial mucosa model at ALI; Fer-1: ferrostatin-1; HTP: heated tobacco product; MFI: mean fluorescent intensity.