FIG. 1.

Fluorescent microscopy of S. cerevisiae cells: I, transformed with the pR1 vector; II, harboring the vector pUR36NLS, showing DNA counterstained with DAPI in blue (A), red fluorescence of DsRed-NLS in red (B), and the overlap of both colors (colocalization) in violet (C); III, harboring the vectors pUR36NLS and pUG34 (IV). Confocal laser scanning microscopy of S. cerevisiae cells during mating. The different mating types are expressing yEGFP3-NLS or DsRed-NLS. The fluorescences of both yEGFP3-NLS and yEGFP3 are coded in green, and those of DsRed-NLS and DsRed are coded in red.