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. 2022 Apr 7;29(10):1996–2008. doi: 10.1038/s41418-022-00991-4

Fig. 6. Enhancement of SDH activity by SIRT3/NIC or TRAP1 parallels inhibition of tumorigenic growth.

Fig. 6

A WB analysis of SIRT3 and SDHA/B protein levels in TRAP1 wild-type (sgEGFP) and knock-out (sgTRAP1) sMPNST cells. Actin was used as a loading control. B Analysis of succinate-coenzyme Q reductase (SQR) activity of SDH in TRAP1 wild-type and knock-out sMPNST cells upon SIRT3 overexpression. SIRT3: cells expressing pFUGW-SIRT3; GFP: cells expressing pFUGW-GFP. C Foci formation of sMPNST cells upon TRAP1 knock-out and SIRT3 overexpression. Effect of NIC treatment (5 mM) on Matrigel colony formation (D), SQR activity (E) and SDHA/B protein levels (F) of TRAP1 wild-type and knock-out sMPNST cells. Measurements in E, F were carried out on cells undergoing tumorigenic growth (day 5th of focus forming assay). In F actin was used as a loading control. Data are reported as mean ± SD values (n ≥ 3); ***p < 0.001; **p < 0.01 and *p < 0.05 with One-way ANOVA followed by Bonferroni post-test.