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. 2022 Sep 30;12:16453. doi: 10.1038/s41598-022-20509-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sterol supplementation study along with cell viability by pretreating the promastigotes with ergosterol/cholesterol followed CA treatment at different time intervals (A). Measurement of ergosterol content in untreated parasites or treated with caprylic acid and AmB (B). Gene expression profiling was studied by semi-quantitative PCR of parasites after treatment with CA and AmB for 12 h (C). Here α-tubulin was used as an internal control.