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. 2022 Sep 30;12:16453. doi: 10.1038/s41598-022-20509-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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10% SDS-PAGE analysis of purified LD-MevK (A). MevK enzyme activity was measured as fold change in NADH oxidation, with CA and its analogues (B). Enzyme activity L. donovani cell extract in presence and absence of CA (C). LB plot of enzyme kinetics assay indicating K_m and V_max (D). Note: **0.05 < P < 0.01; ***0.01 < P < 0.001.