Figure 8.
Evaluation of MevK overexpression in L. donovani after cloning in pLpNeo2 expression vector primers used for confirmation of MevK expression from pLpNeo2 in LD is shown by diagram (A). PCR from transfected and non-transfected cells by primer pair 1–3 and 1–2 (C). Further, semi-quantitative PCR was done to confirm the overexpression (OE) of MevK gene whereα-tubulin was used as an internal control (C). Cell survival with IC50 concentration was measured on transfected and non-transfected cells after treatment with CA (D,E). Note: *P < 0.05.
