Fig. 1.

Analysis of fatty acid consumption by rat-CMs. (a) Protocol used to analyze fatty acid consumption by rat-CMs. Rat-CMs were seeded (day 0) and cultured for 3 days in DMEM-HG medium containing 10% FBS. The medium was changed to rat serum on day 3, and culture was continued for a further 24 h (day 4). Samples of the rat serum used as the medium were obtained before and 24 h after culture for assessment of the lipid profile using gas chromatography. (b) Concentrations of various fatty acids in rat serum before culture (blue) and 24 h after culture (red). Data are shown as mean ± SEM (n = 3 experiments in duplicate). C12:0, lauric acid; C14:0, myristic acid; C14:1, myristoleic acid; C16:0, palmitic acid; C16:1, palmitoleic acid; C18:0, stearic acid; C18:1, oleic acid; C18:2, linoleic acid; C18:3 ω6, ɤ-linoleic acid; C18:3 ω3, linolenic acid; C20:0, arachidic acid; C20:1, eicosenoic acid; C20:2, eicosadienoic acid; C20:3 ω9, 5-8-11 eicosatrienoic acid; C20:3 ω6, dihomo-ɤ-linolenic acid; C20:4, arachidonic acid; C20:5, eicosapentaenoic acid; C22:0, behenic acid; C22:1, erucic acid; C22:4, docosatetraenoic acid; C22:5, docosapentaenoic acid; C24:0, lignoceric acid; C22:6, docosahexaenoic acid; C24:1, nervonic acid. (c) Fatty acid consumption by rat-CMs. Each value is calculated as the difference between the fatty acid content of the serum before culture and the fatty acid content of the serum 24 h after culture. Data are shown as mean ± SEM (n = 3 experiments in duplicate). Abbreviations are as in (b).