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Detection of the KdpFABC complex produced upon exposure of cells to an osmotic upshift imposed by NaCl or CsCl. (A) Autoradiograph of a Western blot of whole-cell extracts developed with anti-KdpB antibodies and 125I-protein A for detection. (B) Graph representing the amounts of KdpFABC synthesized, which were calculated according to a standard curve obtained from known amounts of purified KdpFABC.
